Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

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We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning metabo 15-gauge finish nailer cordless requires two consecutive PCR steps and can be carried out in one day.We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb.EMP further enables simultaneous cloning luce chandelier of multiple inserts.